diff-quik® fix stained smear Search Results


99
Oxford Instruments stain imaris imaging • colocalization • voxel intensity sum
Stain Imaris Imaging • Colocalization • Voxel Intensity Sum, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec inside stain kit
Inside Stain Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Medion Diagnostics diff-quik fix diff-quik
Diff Quik Fix Diff Quik, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Medion Diagnostics giemsa staining diff quik fix
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Giemsa Staining Diff Quik Fix, supplied by Medion Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Becton Dickinson fix/via stain 700
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Fix/Via Stain 700, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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OcellO Inc cooled fix-stain reagent
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Cooled Fix Stain Reagent, supplied by OcellO Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher dapi
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher live/dead fix aqua stain
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Live/Dead Fix Aqua Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dapco Industries fix/stain buffer
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Fix/Stain Buffer, supplied by Dapco Industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Thermo Fisher live dead fixable aqua dead cell stain kit
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Live Dead Fixable Aqua Dead Cell Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Thermo Fisher fix/perm staining kit
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Fix/Perm Staining Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Keygen Biotech calcein acetoxymethyl ester (calcein am)
Activation of phagocytosis by <t>NETs.</t> <t>Neutrophils</t> were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of <t>Giemsa-stained</t> neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.
Calcein Acetoxymethyl Ester (Calcein Am), supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Journal: Frontiers in Immunology

Article Title: Neutrophil Extracellular Traps Activate Proinflammatory Functions of Human Neutrophils

doi: 10.3389/fimmu.2021.636954

Figure Lengend Snippet: Activation of phagocytosis by NETs. Neutrophils were exposed to PMA, NETs, fNETs or left untreated and phagocytosis of fluorescent-labelled particles was assessed by microscopical examination and flow cytometry. (A) Phagocytosis of latex beads was visualized of Giemsa-stained neutrophils by fluorescence microscopy. Size bar=20 µm. (B–D) Representative histogram of neutrophils after phagocytosis of latex beads (B) , opsonized S. aureus bioparticles (C) or opsonized E. coli bioparticles (D) . Signals on the right side of the dashed line in panels B, C and D were considered as phagocytosing cells. Dotted line=neutrophils without particles; bold line=untreated neutrophils; orange=neutrophils exposed to fNETs; blue=neutrophils exposed to NETs. (E–H) Phagocytosis was assessed by analyzing the percent of neutrophils with ingested fluorescent beads (E) and mean fluorescence intensity (MFI) of cells that phagocytosed beads (F) S. aureus (G) or E. coli (H) by flow cytometry. Statistical analysis by ordinary one-way ANOVA with a post hoc Turkey’s test. Asterisks above the bars indicate significance compared to untreated cells. n=3 **=p ≤ 0,01, ***=p ≤ 0,001. n.s., not significant.

Article Snippet: The preparations contained >99% granulocytes, of which >96% were neutrophils and 1%–4% were eosinophils, as determined by Giemsa staining (Diff Quik ® Fix, Medion Diagnostics, Berlin, Germany) of cytocentrifuged (Shandon) samples ( ).

Techniques: Activation Assay, Flow Cytometry, Staining, Fluorescence, Microscopy